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western blot wb  (Bio-Rad)


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    Bio-Rad western blot wb
    Western Blot Wb, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tesee+western+blot/pm41100505-100-7-32?v=Bio-Rad
    Average 95 stars, based on 155 article reviews
    western blot wb - by Bioz Stars, 2026-07
    95/100 stars

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    Analysis of PrP res properties to determine the type of BSE. Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion. WB results show the typical three band pattern of PrP res ; amongst which the upper most band represents di-glycosylated, middle band represents mono-glycosylated and third band represents un-glycosylated PrP. (A) Lane 1; MagicMark (molecular weight marker), Lane 2; C-BSE control, lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE, Lane 6; Negative control. Anti-PrP antibody; 94B4 (a C-terminal antibody that can detect all types of BSE). Results indicate the molecular weight of un-glycosylated PrP res of Canadian BSE case 20 higher than that of the the C-BSE control and consistent with that of the H-BSE control. (B) Lane 1; MagicMark, lane 2; C-BSE control, Lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE control, Lane 6; Negative control. Anti-PrP antibody; P4 (an N - terminal antibody that can detect only H-BSE). Molecular weights are shown in kDa; kilodaltons.

    Journal: Prion

    Article Title: Canadian 2021 H-type Bovine Spongiform Encephalopathy case associated with a novel E211K polymorphism in prion protein gene

    doi: 10.1080/19336896.2025.2511933

    Figure Lengend Snippet: Analysis of PrP res properties to determine the type of BSE. Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion. WB results show the typical three band pattern of PrP res ; amongst which the upper most band represents di-glycosylated, middle band represents mono-glycosylated and third band represents un-glycosylated PrP. (A) Lane 1; MagicMark (molecular weight marker), Lane 2; C-BSE control, lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE, Lane 6; Negative control. Anti-PrP antibody; 94B4 (a C-terminal antibody that can detect all types of BSE). Results indicate the molecular weight of un-glycosylated PrP res of Canadian BSE case 20 higher than that of the the C-BSE control and consistent with that of the H-BSE control. (B) Lane 1; MagicMark, lane 2; C-BSE control, Lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE control, Lane 6; Negative control. Anti-PrP antibody; P4 (an N - terminal antibody that can detect only H-BSE). Molecular weights are shown in kDa; kilodaltons.

    Article Snippet: The Bio-Rad TeSeE ELISA was performed at the network and BSE-RL labs as per the manufacturer’s instructions for manual purification (TeSeE Purification/Detection Kit; Bio-Rad Laboratories).

    Techniques: Western Blot, Molecular Weight, Marker, Control, Negative Control

    Analysis of PrP res properties to determine the type of BSE. Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion. WB results show the typical three band pattern of PrP res ; amongst which the upper most band represents di-glycosylated, middle band represents mono-glycosylated and third band represents un-glycosylated PrP. (A) Lane 1; MagicMark (molecular weight marker), Lane 2; C-BSE control, lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE, Lane 6; Negative control. Anti-PrP antibody; 94B4 (a C-terminal antibody that can detect all types of BSE). Results indicate the molecular weight of un-glycosylated PrP res of Canadian BSE case 20 higher than that of the the C-BSE control and consistent with that of the H-BSE control. (B) Lane 1; MagicMark, lane 2; C-BSE control, Lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE control, Lane 6; Negative control. Anti-PrP antibody; P4 (an N - terminal antibody that can detect only H-BSE). Molecular weights are shown in kDa; kilodaltons.

    Journal: Prion

    Article Title: Canadian 2021 H-type Bovine Spongiform Encephalopathy case associated with a novel E211K polymorphism in prion protein gene

    doi: 10.1080/19336896.2025.2511933

    Figure Lengend Snippet: Analysis of PrP res properties to determine the type of BSE. Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion. WB results show the typical three band pattern of PrP res ; amongst which the upper most band represents di-glycosylated, middle band represents mono-glycosylated and third band represents un-glycosylated PrP. (A) Lane 1; MagicMark (molecular weight marker), Lane 2; C-BSE control, lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE, Lane 6; Negative control. Anti-PrP antibody; 94B4 (a C-terminal antibody that can detect all types of BSE). Results indicate the molecular weight of un-glycosylated PrP res of Canadian BSE case 20 higher than that of the the C-BSE control and consistent with that of the H-BSE control. (B) Lane 1; MagicMark, lane 2; C-BSE control, Lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE control, Lane 6; Negative control. Anti-PrP antibody; P4 (an N - terminal antibody that can detect only H-BSE). Molecular weights are shown in kDa; kilodaltons.

    Article Snippet: Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion.

    Techniques: Western Blot, Molecular Weight, Marker, Control, Negative Control

    Analysis of PrP res properties to determine the type of BSE. Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion. WB results show the typical three band pattern of PrP res ; amongst which the upper most band represents di-glycosylated, middle band represents mono-glycosylated and third band represents un-glycosylated PrP. (A) Lane 1; MagicMark (molecular weight marker), Lane 2; C-BSE control, lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE, Lane 6; Negative control. Anti-PrP antibody; 94B4 (a C-terminal antibody that can detect all types of BSE). Results indicate the molecular weight of un-glycosylated PrP res of Canadian BSE case 20 higher than that of the the C-BSE control and consistent with that of the H-BSE control. (B) Lane 1; MagicMark, lane 2; C-BSE control, Lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE control, Lane 6; Negative control. Anti-PrP antibody; P4 (an N - terminal antibody that can detect only H-BSE). Molecular weights are shown in kDa; kilodaltons.

    Journal: Prion

    Article Title: Canadian 2021 H-type Bovine Spongiform Encephalopathy case associated with a novel E211K polymorphism in prion protein gene

    doi: 10.1080/19336896.2025.2511933

    Figure Lengend Snippet: Analysis of PrP res properties to determine the type of BSE. Results of Bio-Rad TeSeE Hybrid Western blot (WB) on the brain stem at the level of the obex after proteinase K digestion. WB results show the typical three band pattern of PrP res ; amongst which the upper most band represents di-glycosylated, middle band represents mono-glycosylated and third band represents un-glycosylated PrP. (A) Lane 1; MagicMark (molecular weight marker), Lane 2; C-BSE control, lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE, Lane 6; Negative control. Anti-PrP antibody; 94B4 (a C-terminal antibody that can detect all types of BSE). Results indicate the molecular weight of un-glycosylated PrP res of Canadian BSE case 20 higher than that of the the C-BSE control and consistent with that of the H-BSE control. (B) Lane 1; MagicMark, lane 2; C-BSE control, Lane 3; Canadian BSE case 20, Lane 4; H-BSE control, Lane 5; L-BSE control, Lane 6; Negative control. Anti-PrP antibody; P4 (an N - terminal antibody that can detect only H-BSE). Molecular weights are shown in kDa; kilodaltons.

    Article Snippet: Further, to determine the type of BSE, biochemical typing of PK resistant PrP Sc (PrP res ) with Bio-Rad TeSeE Hybrid western blot was performed using anti-PrP 94B4 antibody.

    Techniques: Western Blot, Molecular Weight, Marker, Control, Negative Control